Description: Rabbit polyclonal antibody to PDC-E2 Immunogen: Recombinant full length protein of human PDC-E2 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IF/IC (1/10 - 1/100) Gene Symbol: DLAT Alternative Names: DLTA; Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex mitochondrial; 70 kDa mitochondrial autoantigen of primary biliary cirrhosis; PBC; Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; M2 antigen complex 70 kDa subunit; Pyruvate dehydrogenase complex component E2; PDC-E2; PDCE2Entrez Gene (Human): 1737Entrez Gene (Mouse) : 235339Entrez Gene (Rat) : 81654SwissProt (Human): P10515SwissProt (Mouse) : Q8BMF4SwissProt (Rat) : P08461Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of PDC-E2 expression in HepG2 (A), Jurkat (B), mouse heart (C), rat brain (D), rat kidney (E) whole cell lysates.
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Immunofluorescent analysis of PDC-E2 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.