Description: Rabbit polyclonal antibody to HSD17B13 Immunogen: Recombinant full length protein of human HSD17B13 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100), IF/IC (1/50 - 1/200) Gene Symbol: HSD17B13 Alternative Names: SCDR9; 17-beta-hydroxysteroid dehydrogenase 13; 17-beta-HSD 13; Short-chain dehydrogenase/reductase 9Entrez Gene (Human): 345275Entrez Gene (Mouse) : 243168Entrez Gene (Rat) : 305150SwissProt (Human): Q7Z5P4SwissProt (Mouse) : Q8VCR2SwissProt (Rat) : Q5M875Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of HSD17B13 expression in HepG2 (A), mouse liver (B), mouse kidney (C), rat liver (D) whole cell lysates.
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  Immunohistochemical analysis of HSD17B13 staining in human colorectal cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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  Immunofluorescent analysis of HSD17B13 staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain the cytoplasm (red). DAPI was used to stain the cell nuclei (blue).