Description: Rabbit polyclonal antibody to GIP Immunogen: Recombinant full length protein of human GIP Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: GIP Alternative Names: Gastric inhibitory polypeptide; GIP; Glucose-dependent insulinotropic polypeptide; Incretin hormoneEntrez Gene (Human): 2695Entrez Gene (Mouse) : 14607Entrez Gene (Rat) : 25040SwissProt (Human): P09681SwissProt (Mouse) : P48756SwissProt (Rat) : Q06145Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of GIP expression in HepG2 (A), mouse liver (B), rat liver (C) whole cell lysates.
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Immunohistochemical analysis of GIP staining in rat kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.