Description: Rabbit polyclonal antibody to GW182 Immunogen: KLH-conjugated synthetic peptide of human GW182 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IF/IC (1/50 - 1/200) Gene Symbol: TNRC6A Alternative Names: CAGH26; KIAA1460; TNRC6; Trinucleotide repeat-containing gene 6A protein; CAG repeat protein 26; EMSY interactor protein; GW182 autoantigen; Protein GW1; Glycine-tryptophan protein of 182 kDaEntrez Gene (Human): 27327Entrez Gene (Mouse) : 233833SwissProt (Human): Q8NDV7SwissProt (Mouse) : Q3UHK8Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of GW182 expression in mouse brain (A), rat brain (B) whole cell lysates.
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Immunofluorescent analysis of GW182 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).