Description: Rabbit polyclonal antibody to Pumilio 1 Immunogen: Recombinant full length protein of human Pumilio 1 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/200) Gene Symbol: PUM1 Alternative Names: KIAA0099; PUMH1; Pumilio homolog 1; HsPUM; Pumilio-1Entrez Gene (Human): 9698Entrez Gene (Mouse) : 80912SwissProt (Human): Q14671SwissProt (Mouse) : Q80U78Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of Pumilio 1 expression in K562 (A), NIH3T3 (B) whole cell lysates.
-
Immunofluorescent analysis of Pumilio 1 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).