Description: Rabbit polyclonal antibody to ATG3 Immunogen: Recombinant full length protein of human ATG3 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/200) Gene Symbol: ATG3 Alternative Names: APG3; APG3L; Ubiquitin-like-conjugating enzyme ATG3; Autophagy-related protein 3; APG3-like; hApg3; Protein PC3-96Entrez Gene (Human): 64422Entrez Gene (Mouse) : 67841Entrez Gene (Rat) : 171415SwissProt (Human): Q9NT62SwissProt (Mouse) : Q9CPX6SwissProt (Rat) : Q6AZ50Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of ATG3 expression in Raji (A), Hela (B), mouse liver (C), mouse heart (D) whole cell lysates.
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Immunofluorescent analysis of ATG3 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).