Description: Rabbit polyclonal antibody to PHAP1 Immunogen: Recombinant full length protein of human PHAP1 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: ANP32A Alternative Names: C15orf1; LANP; MAPM; PHAP1; Acidic leucine-rich nuclear phosphoprotein 32 family member A; Acidic nuclear phosphoprotein pp32; pp32; Leucine-rich acidic nuclear protein; LANP; Mapmodulin; Potent heat-stable protein phosphatase 2A inhibitor I1PP2A; Putative HLA-DR-associated protein I; PHAPIEntrez Gene (Human): 8125Entrez Gene (Mouse) : 11737Entrez Gene (Rat) : 25379SwissProt (Human): P39687SwissProt (Mouse) : O35381SwissProt (Rat) : P49911Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of PHAP1 expression in HepG2 (A), MCF7 (B), mouse lung (C), mouse brain (D) whole cell lysates.
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Immunohistochemical analysis of PHAP1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.