Anti-HPRT Antibody 
Datasheet
MSDS
Description: Rabbit polyclonal antibody to HPRT Immunogen: Recombinant full length protein of human HPRT Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IF/IC (1/50 - 1/200) Gene Symbol: HPRT1 Alternative Names: HPRT; Hypoxanthine-guanine phosphoribosyltransferase; HGPRT; HGPRTaseEntrez Gene (Human): 3251Entrez Gene (Mouse) : 15452Entrez Gene (Rat) : 24465SwissProt (Human): P00492SwissProt (Mouse) : P00493SwissProt (Rat) : P27605Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of HPRT expression in SW480 (A), HT29 (B), Hela (C), mouse brain (D), mouse liver (E) whole cell lysates. -
Immunofluorescent analysis of HPRT staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Oral exposure to inorganic mercury or methylmercury elicits distinct pro-inflammatory and pro-oxidant intestinal responses in a mouse model system
Estrategias para mitigar la toxicidad asociada a la exposición crónica al mercurio a través de la dieta
Protective effects of oral administration of lactic acid bacteria strains against methylmercury-induced intestinal toxicity in a murine model
Lactobacillus strains reduce the toxic effects of a subchronic exposure to arsenite through drinking water