Description: Rabbit polyclonal antibody to EVI1 Immunogen: Recombinant full length protein of human EVI1 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: MECOM Alternative Names: EVI1; MDS1 and EVI1 complex locus protein EVI1; Ecotropic virus integration site 1 protein homolog; EVI-1Entrez Gene (Human): 2122SwissProt (Human): Q03112SwissProt (Mouse) : P14404Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of EVI1 expression in mouse kidney (A), mouse heart (B) whole cell lysates.
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Immunohistochemical analysis of EVI1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.