Description: Rabbit polyclonal antibody to HAX1 Immunogen: Recombinant full length protein of human HAX1 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/200) Gene Symbol: HAX1 Alternative Names: HS1BP1; HCLS1-associated protein X-1; HS1-associating protein X-1; HAX-1; HS1-binding protein 1; HSP1BP-1Entrez Gene (Human): 10456Entrez Gene (Mouse) : 23897Entrez Gene (Rat) : 291202SwissProt (Human): O00165SwissProt (Mouse) : O35387SwissProt (Rat) : Q7TSE9Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of HAX1 expression in MCF7 (A), HepG2 (B), mouse kidney (C), mouse brain (D) whole cell lysates.
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Immunofluorescent analysis of HAX1 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.