Description: Rabbit polyclonal antibody to NBC1 Immunogen: Recombinant full length protein of human NBC1 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/200) Gene Symbol: SLC4A4 Alternative Names: NBC; NBC1; NBCE1; Electrogenic sodium bicarbonate cotransporter 1; Sodium bicarbonate cotransporter; Na(+)/HCO3(-) cotransporter; Solute carrier family 4 member 4; kNBC1Entrez Gene (Human): 8671Entrez Gene (Mouse) : 54403Entrez Gene (Rat) : 84484SwissProt (Human): Q9Y6R1SwissProt (Mouse) : O88343SwissProt (Rat) : Q9JI66Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

• 
  Western blot analysis of NBC1 expression in PC3 (A), A549 (B) whole cell lysates.
• 
  Immunofluorescent analysis of NBC1 staining in C6 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).