Description: Rabbit polyclonal antibody to Atlastin-1 Immunogen: Recombinant full length protein of human Atlastin-1 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IF/IC (1/50 - 1/200) Gene Symbol: ATL1 Alternative Names: GBP3; SPG3A; Atlastin-1; Brain-specific GTP-binding protein; GTP-binding protein 3; GBP-3; hGBP3; Guanine nucleotide-binding protein 3; Spastic paraplegia 3 protein AEntrez Gene (Human): 51062Entrez Gene (Mouse) : 73991Entrez Gene (Rat) : 362750SwissProt (Human): Q8WXF7SwissProt (Mouse) : Q8BH66SwissProt (Rat) : Q6PST4Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Atlastin-1 expression in LOVO (A), HEK293T (B), mouse brain (C) whole cell lysates.
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Immunofluorescent analysis of Atlastin-1 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.