Description: Rabbit polyclonal antibody to ABAT Immunogen: Recombinant full length protein of human ABAT Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/200) Gene Symbol: ABAT Alternative Names: GABAT; 4-aminobutyrate aminotransferase mitochondrial; (S)-3-amino-2-methylpropionate transaminase; GABA aminotransferase; GABA-AT; Gamma-amino-N-butyrate transaminase; GABA transaminase; GABA-T; L-AIBATEntrez Gene (Human): 18Entrez Gene (Mouse) : 268860Entrez Gene (Rat) : 81632SwissProt (Human): P80404SwissProt (Mouse) : P61922SwissProt (Rat) : P50554Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of ABAT expression in SW480 (A), mouse kidney (B), mouse brain (C) whole cell lysates.
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Immunofluorescent analysis of ABAT staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.