Description: Rabbit polyclonal antibody to eIF4A1 Immunogen: Recombinant full length protein of human eIF4A1 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IF/IC (1/50 - 1/200), IP (1/20 - 1/50) Gene Symbol: EIF4A1 Alternative Names: DDX2A; EIF4A; Eukaryotic initiation factor 4A-I; eIF-4A-I; eIF4A-I; ATP-dependent RNA helicase eIF4A-1Entrez Gene (Human): 1973Entrez Gene (Mouse) : 13681SwissProt (Human): P60842SwissProt (Mouse) : P60843Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

• 
  Western blot analysis of eIF4A1 expression in Hela (A), Jurkat (B), NIH3T3 (C), PC12 (D), mouse liver (E) whole cell lysates.
• 
  Immunofluorescent analysis of eIF4A1 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.