Description: Rabbit polyclonal antibody to Tropomyosin 2 Immunogen: KLH-conjugated synthetic peptide of human Tropomyosin 2 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200) Gene Symbol: TPM2 Alternative Names: TMSB; Tropomyosin beta chain; Beta-tropomyosin; Tropomyosin-2Entrez Gene (Human): 7169Entrez Gene (Mouse) : 22004Entrez Gene (Rat) : 500450SwissProt (Human): P07951SwissProt (Mouse) : P58774SwissProt (Rat) : P58775Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Tropomyosin 2 expression in Hela (A), MCF7 (B), mouse lung (C) whole cell lysates.
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Immunohistochemical analysis of Tropomyosin 2 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.