Description: Rabbit polyclonal antibody to ASCT1 Immunogen: KLH-conjugated synthetic peptide of human ASCT1 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: SLC1A4 Alternative Names: ASCT1; SATT; Neutral amino acid transporter A; Alanine/serine/cysteine/threonine transporter 1; ASCT-1; SATT; Solute carrier family 1 member 4Entrez Gene (Human): 6509Entrez Gene (Mouse) : 55963SwissProt (Human): P43007SwissProt (Mouse) : O35874Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of ASCT1 expression in HepG2 (A) whole cell lysates.
-
Immunohistochemical analysis of ASCT1 staining in human ovarian cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.