Description: Rabbit polyclonal antibody to PSMA2 Immunogen: Recombinant full length protein of human PSMA2 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/200) Gene Symbol: PSMA2 Alternative Names: HC3; PSC3; Proteasome subunit alpha type-2; Macropain subunit C3; Multicatalytic endopeptidase complex subunit C3; Proteasome component C3Entrez Gene (Human): 5683Entrez Gene (Mouse) : 19166Entrez Gene (Rat) : 29669SwissProt (Human): P25787SwissProt (Mouse) : P49722SwissProt (Rat) : P17220Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of PSMA2 expression in Jurkat (A), Hela (B), NIH3T3 (C), mouse brain (D) whole cell lysates.
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Immunofluorescent analysis of PSMA2 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.