Description: Rabbit polyclonal antibody to Dnmt3L Immunogen: Recombinant full length protein of human Dnmt3L Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: DNMT3L Alternative Names: DNA cytosine-5)-methyltransferase 3-likeEntrez Gene (Human): 29947Entrez Gene (Mouse) : 54427Entrez Gene (Rat) : 309680SwissProt (Human): Q9UJW3SwissProt (Mouse) : Q9CWR8SwissProt (Rat) : Q1LZ50Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Dnmt3L expression in PC3 (A), Jurkat (B), MCF7 (C) whole cell lysates.
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Immunohistochemical analysis of Dnmt3L staining in rat testis formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.