Description: Rabbit polyclonal antibody to EDF1 Immunogen: Recombinant full length protein of human EDF1 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: EDF1 Alternative Names: Endothelial differentiation-related factor 1; EDF-1; Multiprotein-bridging factor 1; MBF1Entrez Gene (Human): 8721Entrez Gene (Mouse) : 59022Entrez Gene (Rat) : 296570SwissProt (Human): O60869SwissProt (Mouse) : Q9JMG1SwissProt (Rat) : P69736Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of EDF1 expression in HepG2 (A), mouse brain (B), rat brain (C) whole cell lysates.
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  Immunohistochemical analysis of EDF1 staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.