Description: Rabbit polyclonal antibody to PPX Immunogen: Recombinant full length protein of human PPX Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200), IP (1/10 - 1/50) Gene Symbol: PPP4C Alternative Names: PPP4; PPX; Serine/threonine-protein phosphatase 4 catalytic subunit; PP4C; Pp4; Protein phosphatase X; PP-XEntrez Gene (Human): 5531Entrez Gene (Mouse) : 56420Entrez Gene (Rat) : 171366SwissProt (Human): P60510SwissProt (Mouse) : P97470SwissProt (Rat) : Q5BJ92Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of PPX expression in MCF7 (A), NIH3T3 (B), mouse testis (C), mouse spleen (D) whole cell lysates.
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Immunohistochemical analysis of PPX staining in rat lung formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.