Description: Rabbit polyclonal antibody to Karyopherin alpha-4 Immunogen: Recombinant full length protein of human Karyopherin alpha-4 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200) Gene Symbol: KPNA4 Alternative Names: QIP1; Importin subunit alpha-3; Importin alpha Q1; Qip1; Karyopherin subunit alpha-4Entrez Gene (Human): 3840Entrez Gene (Mouse) : 16649SwissProt (Human): O00629SwissProt (Mouse) : O35343Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Karyopherin alpha-4 expression in A549 (A), SKOV3 (B) whole cell lysates.
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  Immunohistochemical analysis of Karyopherin alpha-4 staining in human esophageal cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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  Immunofluorescent analysis of Karyopherin alpha-4 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).