Description: Rabbit polyclonal antibody to Histone H2B Immunogen: Recombinant full length protein of human Histone H2B Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: HIST2H2BE Alternative Names: H2BFQ; Histone H2B type 2-E; Histone H2B-GL105; Histone H2B.q; H2B/qEntrez Gene (Human): 8349Entrez Gene (Mouse) : 319190SwissProt (Human): Q16778SwissProt (Mouse) : Q64524Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Histone H2B expression in SW480 (A), BT474 (B) whole cell lysates.
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Immunohistochemical analysis of Histone H2B staining in human esophageal cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.