Description: Rabbit polyclonal antibody to Glyoxalase I Immunogen: Recombinant full length protein of human Glyoxalase I Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: GLO1 Alternative Names: Lactoylglutathione lyase; Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyaseEntrez Gene (Human): 2739Entrez Gene (Mouse) : 109801Entrez Gene (Rat) : 294320SwissProt (Human): Q04760SwissProt (Mouse) : Q9CPU0SwissProt (Rat) : Q6P7Q4Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Glyoxalase I expression in 22RV1 (A), PC3 (B), mouse kidney (C) whole cell lysates.
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  Immunohistochemical analysis of Glyoxalase I staining in mouse kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.