Description: Rabbit polyclonal antibody to Optineurin Immunogen: Recombinant full length protein of human Optineurin Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: OPTN Alternative Names: FIP2; GLC1E; HIP7; HYPL; NRP; Optineurin; E3-14.7K-interacting protein; FIP-2; Huntingtin yeast partner L; Huntingtin-interacting protein 7; HIP-7; Huntingtin-interacting protein L; NEMO-related protein; Optic neuropathy-inducing protein; Transcription factor IIIA-interacting protein; TFIIIA-IntPEntrez Gene (Human): 10133Entrez Gene (Mouse) : 71648SwissProt (Human): Q96CV9SwissProt (Mouse) : Q8K3K8Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Optineurin expression in Hela (A), A549 (B), mouse liver (C) whole cell lysates.
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Immunohistochemical analysis of Optineurin staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.