Description: Rabbit polyclonal antibody to CD158i Immunogen: Recombinant full length protein of human CD158i Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IF/IC (1/50 - 1/200) Gene Symbol: KIR2DS4 Alternative Names: CD158I; KKA3; NKAT8; Killer cell immunoglobulin-like receptor 2DS4; CD158 antigen-like family member I; MHC class I NK cell receptor; Natural killer-associated transcript 8; NKAT-8; P58 natural killer cell receptor clones CL-39/CL-17; p58 NK receptor CL-39/CL-17; CD158iEntrez Gene (Human): 3809SwissProt (Human): P43632Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of CD158i expression in HEK293T (A) whole cell lysates.
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  Immunofluorescent analysis of CD158i staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).