Description: Rabbit polyclonal antibody to PSMB9 Immunogen: Recombinant full length protein of human PSMB9 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/200) Gene Symbol: PSMB9 Alternative Names: LMP2; PSMB6i; RING12; Proteasome subunit beta type-9; Low molecular mass protein 2; Macropain chain 7; Multicatalytic endopeptidase complex chain 7; Proteasome chain 7; Proteasome subunit beta-1i; Really interesting new gene 12 proteinEntrez Gene (Human): 5698Entrez Gene (Mouse) : 16912SwissProt (Human): P28065SwissProt (Mouse) : P28076Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of PSMB9 expression in HepG2 (A), THP1 (B), mouse liver (C) whole cell lysates.
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  Immunofluorescent analysis of PSMB9 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.