Description: Rabbit polyclonal antibody to E6AP Immunogen: Recombinant full length protein of human E6AP Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: UBE3A Alternative Names: E6AP; EPVE6AP; HPVE6A; Ubiquitin-protein ligase E3A; E6AP ubiquitin-protein ligase; Human papillomavirus E6-associated protein; Oncogenic protein-associated protein E6-AP; Renal carcinoma antigen NY-REN-54Entrez Gene (Human): 7337SwissProt (Human): Q05086SwissProt (Mouse) : O08759Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of E6AP expression in SKOV3 (A), Jurkat (B), mouse brain (C), rat brain (D) whole cell lysates.
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  Immunohistochemical analysis of E6AP staining in rat kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.