Description: Rabbit polyclonal antibody to Frataxin Immunogen: Recombinant full length protein of human Frataxin Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: FXN Alternative Names: FRDA; X25; Frataxin mitochondrial; Friedreich ataxia protein; FxnEntrez Gene (Human): 2395Entrez Gene (Mouse) : 14297SwissProt (Human): Q16595SwissProt (Mouse) : O35943SwissProt (Rat) : D3ZYW7Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Frataxin expression in mouse liver (A), rat kidney (B) whole cell lysates.
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Immunohistochemical analysis of Frataxin staining in rat heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.