Description: Rabbit polyclonal antibody to PSMC5 Immunogen: Recombinant full length protein of human PSMC5 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/200) Gene Symbol: PSMC5 Alternative Names: SUG1; 26S protease regulatory subunit 8; 26S proteasome AAA-ATPase subunit RPT6; Proteasome 26S subunit ATPase 5; Proteasome subunit p45; Thyroid hormone receptor-interacting protein 1; TRIP1; p45/SUGEntrez Gene (Human): 5705Entrez Gene (Mouse) : 19184Entrez Gene (Rat) : 81827SwissProt (Human): P62195SwissProt (Mouse) : P62196SwissProt (Rat) : P62198Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of PSMC5 expression in HepG2 (A), MCF7 (B), NIH3T3 (C), PC12 (D) whole cell lysates.
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Immunofluorescent analysis of PSMC5 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.