Description: Rabbit polyclonal antibody to CARD4 Immunogen: Recombinant full length protein of human CARD4 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: NOD1 Alternative Names: CARD4; Nucleotide-binding oligomerization domain-containing protein 1; Caspase recruitment domain-containing protein 4Entrez Gene (Human): 10392Entrez Gene (Mouse) : 107607SwissProt (Human): Q9Y239SwissProt (Mouse) : Q8BHB0Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of CARD4 expression in mouse lung (A) whole cell lysates.
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  Immunohistochemical analysis of CARD4 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.