Description: Rabbit polyclonal antibody to GGA2 Immunogen: Recombinant full length protein of human GGA2 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/200) Gene Symbol: GGA2 Alternative Names: KIAA1080; ADP-ribosylation factor-binding protein GGA2; Gamma-adaptin-related protein 2; Golgi-localized, gamma ear-containing, ARF-binding protein 2; VHS domain and ear domain of gamma-adaptin; VearEntrez Gene (Human): 23062Entrez Gene (Mouse) : 74105SwissProt (Human): Q9UJY4SwissProt (Mouse) : Q6P5E6Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of GGA2 expression in HepG2 (A), Raji (B), MCF7 (C), mouse brain (D) whole cell lysates.
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Immunofluorescent analysis of GGA2 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.