Description: Rabbit polyclonal antibody to Fibrillarin Immunogen: Recombinant full length protein of human Fibrillarin Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IF/IC (1/50 - 1/200), IP (1/20 - 1/50) Gene Symbol: FBL Alternative Names: FIB1; FLRN; rRNA 2'-O-methyltransferase fibrillarin; 34 kDa nucleolar scleroderma antigen; Histone-glutamine methyltransferaseEntrez Gene (Human): 2091Entrez Gene (Mouse) : 102643269; 14113Entrez Gene (Rat) : 292747SwissProt (Human): P22087SwissProt (Mouse) : P35550SwissProt (Rat) : P22509Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Fibrillarin expression in Hela (A), U20S (B), COS7 (C), PC12 (D) whole cell lysates.
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Immunofluorescent analysis of Fibrillarin staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.