Description: Rabbit polyclonal antibody to HADH Immunogen: Recombinant full length protein of human HADH Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: HADH Alternative Names: HAD; HADHSC; SCHAD; Hydroxyacyl-coenzyme A dehydrogenase mitochondrial; HCDH; Medium and short-chain L-3-hydroxyacyl-coenzyme A dehydrogenase; Short-chain 3-hydroxyacyl-CoA dehydrogenaseEntrez Gene (Human): 3033Entrez Gene (Mouse) : 15107Entrez Gene (Rat) : 113965SwissProt (Human): Q16836SwissProt (Mouse) : Q61425SwissProt (Rat) : Q9WVK7Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of HADH expression in Jurkat (A), HT29 (B), HL60 (C) whole cell lysates.
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Immunohistochemical analysis of HADH staining in rat liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.