Description: Rabbit polyclonal antibody to Alpha-tubulin 4a Immunogen: Recombinant full length protein of human Alpha-tubulin 4a Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IF/IC (1/10 - 1/100) Gene Symbol: TUBA4A Alternative Names: TUBA1; Tubulin alpha-4A chain; Alpha-tubulin 1; Testis-specific alpha-tubulin; Tubulin H2-alpha; Tubulin alpha-1 chainEntrez Gene (Human): 7277Entrez Gene (Mouse) : 22145Entrez Gene (Rat) : 316531SwissProt (Human): P68366SwissProt (Mouse) : P68368SwissProt (Rat) : Q5XIF6Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Alpha-tubulin 4a expression in LOVO (A), HEK293T (B), HUVEC (C) whole cell lysates.
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Immunofluorescent analysis of Alpha-tubulin 4a staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.