Description: Rabbit polyclonal antibody to OLFM1 Immunogen: Recombinant full length protein of human OLFM1 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: OLFM1 Alternative Names: NOE1; NOEL1; Noelin; Neuronal olfactomedin-related ER localized protein; Olfactomedin-1Entrez Gene (Human): 10439SwissProt (Human): Q99784Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of OLFM1 expression in Hela (A), HepG2 (B) whole cell lysates. (Predicted band size: 15-17; 52-55 kD; Observed band size: 55 kD)
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Immunohistochemical analysis of OLFM1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.