Description: Rabbit polyclonal antibody to Calpain 1 Immunogen: Recombinant full length protein of human Calpain 1 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: CAPN1 Alternative Names: CANPL1; Calpain-1 catalytic subunit; Calcium-activated neutral proteinase 1; CANP 1; Calpain mu-type; Calpain-1 large subunit; Cell proliferation-inducing gene 30 protein; Micromolar-calpain; muCANPEntrez Gene (Human): 823Entrez Gene (Mouse) : 12333Entrez Gene (Rat) : 29153SwissProt (Human): P07384SwissProt (Mouse) : O35350SwissProt (Rat) : P97571Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Calpain 1 expression in A549 (A), MCF7 (B), Jurkat (C) whole cell lysates.
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  Immunohistochemical analysis of Calpain 1 staining in mouse heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.