Description: Mouse monoclonal antibody to CD105 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human CD105. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Mouse IgG2b. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/300) Gene Symbol: ENG Alternative Names: END; Endoglin; CD105Entrez Gene (Human): 2022SwissProt (Human): P17813Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Immunohistochemical analysis of CD105 staining in human placenta formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Immunohistochemical analysis of CD105 staining in human renal clear cell carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Immunohistochemical analysis of CD105 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.