Description: Mouse monoclonal antibody to Collagen 3 alpha 1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human Collagen 3 alpha 1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Mouse IgG2a. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/300), IF (1/100 - 1/500) Gene Symbol: COL3A1 Alternative Names: Collagen alpha-1(III) chainEntrez Gene (Human): 1281SwissProt (Human): P02461Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Collagen 3 alpha 1 expression in Hela (A) whole cell lysates.
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  Immunohistochemical analysis of Collagen 3 alpha 1 staining in human skin formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.