Description: Mouse monoclonal antibody to Villin Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human Villin. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Mouse IgG2b. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/300) Gene Symbol: VIL1 Alternative Names: VIL; Villin-1Entrez Gene (Human): 7429SwissProt (Human): P09327Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Villin expression in HepG2 (A), SW480 (B) whole cell lysates.
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  Immunohistochemical analysis of Villin staining in human appendix formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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  Immunohistochemical analysis of Villin staining in human colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.