Description: Mouse monoclonal antibody to Fascin Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human Fascin. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Mouse IgG1. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/300) Gene Symbol: FSCN1 Alternative Names: FAN1; HSN; SNL; Fascin; 55 kDa actin-bundling protein; Singed-like protein; p55Entrez Gene (Human): 6624SwissProt (Human): Q16658Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Fascin expression in Hela (A), HepG2 (B), K562 (C) whole cell lysates.
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  Immunohistochemical analysis of Fascin staining in human classical hodgkin's lymphoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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  Immunohistochemical analysis of Fascin staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.