Description: Mouse monoclonal antibody to Cytokeratin 17 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human Cytokeratin 17. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Mouse IgG1. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/300) Gene Symbol: KRT17 Alternative Names: Keratin type I cytoskeletal 17; 39.1; Cytokeratin-17; CK-17; Keratin-17; K17Entrez Gene (Human): 3872SwissProt (Human): Q04695Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Cytokeratin 17 expression in Hela (A), A431 (B) whole cell lysates.
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Immunohistochemical analysis of Cytokeratin 17 staining in human lung squamous carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Immunohistochemical analysis of Cytokeratin 17 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.