Description: Mouse monoclonal antibody to Actin pan Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human Actin pan. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Mouse IgG2a. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/300) Gene Symbol: ACTG1 Alternative Names: ACTB; Actin cytoplasmic 1; Beta-actin; POTEKP; ACTBL3; FKSG30; Putative beta-actin-like protein 3; Kappa-actin; POTE ankyrin domain family member K; ACTG1; ACTB; ACTG; Actin cytoplasmic 2; Gamma-actinEntrez Gene (Human): 60; 71SwissProt (Human): P60709; Q9BYX7; P63261Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Actin pan expression in Hela (A), HepG2 (B), MCF7 (C) whole cell lysates.
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  Immunohistochemical analysis of Actin pan staining in human cardiac muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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  Immunohistochemical analysis of Actin pan staining in human colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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  Immunohistochemical analysis of Actin pan staining in human skeletal muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.