Description: Mouse monoclonal antibody to Von Hippel Lindau Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human Von Hippel Lindau. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Mouse IgG3. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide. Dilution: IH (1/100 - 1/300) Gene Symbol: VHL Alternative Names: Von Hippel-Lindau disease tumor suppressor; Protein G7; pVHLEntrez Gene (Human): 7428SwissProt (Human): P40337Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Immunohistochemical analysis of Von Hippel Lindau staining in human kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.