Description: Mouse monoclonal antibody to PCNA Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human PCNA. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Mouse IgG1. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/300) Gene Symbol: PCNA Alternative Names: Proliferating cell nuclear antigen; PCNA; CyclinEntrez Gene (Human): 5111SwissProt (Human): P12004Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of PCNA expression in K562 (A), HEK293 (B), A431 (C) whole cell lysates.
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  Immunohistochemical analysis of PCNA staining in human colon carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.