Description: Mouse monoclonal antibody to CD99 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human CD99. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Mouse IgG. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide. Dilution: IH (1/100 - 1/300) Gene Symbol: CD99 Alternative Names: MIC2; MIC2X; MIC2Y; CD99 antigen; 12E7; E2 antigen; Protein MIC2; T-cell surface glycoprotein E2; CD99Entrez Gene (Human): 4267SwissProt (Human): P14209Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Immunohistochemical analysis of CD99 staining in human ewing's sarcoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Identification and validation of a novel ubiquitination-related gene UBE2T in Ewing's sarcoma