Description: Rabbit polyclonal antibody to Vinculin Immunogen: KLH-conjugated synthetic peptide encompassing a sequence of human Vinculin. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/1000 - 1/2000), IH (1/200 - 1/500) Gene Symbol: VCL Alternative Names: Vinculin; Metavinculin; MVEntrez Gene (Human): 7414Entrez Gene (Mouse) : 22330Entrez Gene (Rat) : 305679SwissProt (Human): P18206SwissProt (Mouse) : Q64727SwissProt (Rat) : P85972Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Vinculin expression in Hela (A), K562 (B), mouse brain (C), rat brain (D) whole cell lysates.
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  Immunohistochemical analysis of Vinculin staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.