Description: Rabbit polyclonal antibody to STAT5B Immunogen: Recombinant protein corresponding to human STAT5B. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/1000 - 1/2000), IH (1/200 - 1/500) Gene Symbol: STAT5B Alternative Names: Signal transducer and activator of transcription 5BEntrez Gene (Human): 6777SwissProt (Human): P51692Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of STAT5B expression in K562 (A), Hela (B), NIH3T3 (C), mouse heart (D), PC12 (E), rat heart (F) whole cell lysates.
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Immunohistochemical analysis of STAT5B staining in human spleen formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.