Description: Rabbit polyclonal antibody to SOD2 Immunogen: Recombinant protein corresponding to human SOD2. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/1000 - 1/2000), IH (1/200 - 1/500) Gene Symbol: SOD2 Alternative Names: Superoxide dismutase [Mn] mitochondrialEntrez Gene (Human): 6648SwissProt (Human): P04179Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of SOD2 expression in 293T (A), Hela (B), C2C12 (C), NIH3T3 (D), rat liver (E), rat brain (F) whole cell lysates.
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Immunohistochemical analysis of SOD2 staining in mouse kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.