Description: Rabbit polyclonal antibody to S100-B Immunogen: Recombinant protein corresponding to human S100-B. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/1000 - 1/2000), IH (1/50 - 1/200) Gene Symbol: S100B Alternative Names: Protein S100-B; S-100 protein beta chain; S-100 protein subunit beta; S100 calcium-binding protein BEntrez Gene (Human): 6285SwissProt (Human): P04271Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of S100-B expression in mouse brain (A), rat brain (B) whole cell lysates.
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Immunohistochemical analysis of S100-B staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.