Description: Rabbit polyclonal antibody to MKK5 Immunogen: Recombinant protein corresponding to human MKK5. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/1000 - 1/2000), IH (1/200 - 1/500) Gene Symbol: MAP2K5 Alternative Names: MEK5; MKK5; PRKMK5; Dual specificity mitogen-activated protein kinase kinase 5; MAP kinase kinase 5; MAPKK 5; MAPK/ERK kinase 5; MEK 5Entrez Gene (Human): 5067SwissProt (Human): Q13163Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of MKK5 expression in Hela (A), NIH3T3 (B), PC12 (C) whole cell lysates.
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Immunohistochemical analysis of MKK5 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.